Søgeord (borrelia) valgt.
21 emner vises.
1
Exploring the dynamics of Borrelia burgdorferi sensu lato antibodies – a registry-based study on laboratory data from Sweden and Denmark
Marc Westerholt, Karen Angeliki Krogfelt, Ram Benny Dessau, Lukas Frans Ocias
Clinical Microbiology and Infection, 26.09.2023
Tilføjet 26.09.2023
Lyme borreliosis (LB) is the most common tick-transmitted infection on the northern hemisphere and is caused by bacteria in the Borrelia burgdorferi sensu lato (Bbsl)-complex. The diagnosis is partially based on serology and clinicians often take follow-up serum samples to look for seroconversion or an increase in IgG-antibody levels. In this registry-based study, we proposed a method for determining actual changes in IgG and examined antibody reactivity and decay.
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2
Synopsis - Characteristics of Hard Tick Relapsing Fever Caused by Borrelia miyamotoi, United States, 2013-2019
Emerging Infectious Diseases, 19.08.2023
Tilføjet 19.08.2023
Ongoing surveillance will improve understanding of the incidence, clinical severity, and public health role of this emerging disease.
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3
Seroprevalence and risk factors of Borrelia burgdorferi sensu lato and Rickettsia species infection in humans in Mongolia, 2016–2020
Dashdavaa Ganbold, Bayarsaikhan Uudus, Naranbat Nyamdavaa, Yeruult Chultemsuren, Amarbayasgalan Zagd, Mungunzaya Tangad, Agarzandan Bayarmaa, Rolomjav Lkunrev, Uyanga Baasandagva, Tsogbadrakh Nyamdorj, Myadagsuren Narankhajid
PLoS One Infectious Diseases, 9.08.2023
Tilføjet 9.08.2023
by Dashdavaa Ganbold, Bayarsaikhan Uudus, Naranbat Nyamdavaa, Yeruult Chultemsuren, Amarbayasgalan Zagd, Mungunzaya Tangad, Agarzandan Bayarmaa, Rolomjav Lkunrev, Uyanga Baasandagva, Tsogbadrakh Nyamdorj, Myadagsuren Narankhajid Borrelia burgdorferi sensu lato and Rickettsia spp. are worldwide causes of tick-borne infections. We aimed to estimate the seroprevalence of immunoglobulin G (IgG) antibodies against different tick-borne diseases (TBDs) and determine risk factors among Mongolians from 2016 to 2020. Blood samples were obtained from voluntary participants with a history of suspected tick bite who visited our hospital, and IgG antibodies against Rickettsia and Borrelia were detected using enzyme-linked immunosorbent assay (ELISA). The IgG antibody seropositivity rate against Rickettsia was 21.8% (1032/4724), while 3.4% (162/4724) of participants tested positive for serum IgG antibodies against Borrelia by ELISA.Binary logistic regression analysis was performed to evaluate risk factors for tick-borne rickettsiosis (TBR) and tick-borne borreliosis (TBB) using IgG serum sample. Age, occupation, and residence were significantly associated with these diseases; however, sex did not show any significant association. Seroprevalence was significantly higher among herders (40.6%, 95% confidence interval [CI]: 35.5–45.8; odds ratio [OR] 0.61; P < 0.001) and students (32.8%, 95% CI: 30.2–35.4; OR 0.75; P < 0.001) than among individuals with other occupations. The 25–29 age group had a slightly higher seroprevalence (35.1%, 95% CI: 28.1–42.6; OR 0.61; P < 0.006) than those in other age groups. Province was a stronger predictor of TBR than occupation and age group. In univariate subgroup analysis by age group, occupation, and residence were significantly associated with TBR seroprevalence, whereas age and province were associated with TBB seroprevalence. Thus, risk factors for TBD include residence, occupation, and age group. This study was conducted using samples from all Mongolian provinces and the capital city, and the risk factors and prevalence of Rickettsia and Borreliaare highlighted.
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4
Identification of potential biomarkers in active Lyme borreliosis
Ivar Tjernberg, Malin Lager, Guro Furset Jensen, Randi Eikeland, Dag Nyman, Lars Brudin, Anna J. Henningsson
PLoS One Infectious Diseases, 26.06.2023
Tilføjet 26.06.2023
by Ivar Tjernberg, Malin Lager, Guro Furset Jensen, Randi Eikeland, Dag Nyman, Lars Brudin, Anna J. Henningsson Objectives Lyme serology does not readily discriminate an active Lyme borreliosis (LB) from a previous Borrelia infection or exposure. Here, we aimed to investigate a large number of immunological protein biomarkers to search for an immunological pattern typical for active LB, in contrast to patterns found in healthy blood donors, a proportion of whom were previously exposed to Borrelia. Methods Serum samples from well-characterised adult patients with ongoing LB and healthy blood donors were included and investigated using a proximity extension assay (provided by Olink®) by which 92 different immune response-related human protein biomarkers were analysed simultaneously. Results In total, 52 LB patients and 75 healthy blood donors were included. The blood donors represented both previously Borrelia exposed (n = 34) and not exposed (n = 41) based on anti-Borrelia antibody status. Ten of the examined 92 proteins differed between patients and blood donors and were chosen for further logistic regression (p
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5
Which trial do we need? Treatment of multiple erythema migrans with doxycycline for 7 days versus 14 days
Daša Stupica, Stefan Collinet-Adler, Nataša Kejžar, Maša Velušček, Katarina Boršič
Clinical Microbiology and Infection, 21.06.2023
Tilføjet 21.06.2023
Lyme borreliosis is the most prevalent vector-borne disease in Europe and North America [1]; an estimated ≈232,000 cases in Western Europe [2] and ≈476,000 cases in the US [3] are diagnosed per year and erythema migrans is its most frequent clinical presentation, occurring in ≥80% of cases [1,4]. In solitary erythema migrans (SEM), the skin manifestation remains localised to the site of inoculation of borreliae, whereas multiple erythema migrans (MEM) represent hematogenous dissemination of borreliae, which may occur in 13.4% to 27% of US cases [5‒7] and less frequently (up to 7%) in Europe [4,6].
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6
A case report of unilateral cerebral vasculitis in adults: keep in mind Lyme neuroborreliosis
BMC Infectious Diseases, 5.05.2023
Tilføjet 5.05.2023
Abstract Background Lyme neuroborreliosis (LNB), due to infection of the nervous system by the spirochete Borrelia burgdorferi, occurs in 15% of Lyme disease cases. However, neurovascular involvement is uncommon, especially recurrent stroke related to cerebral vasculitis in the absence of CSF pleocytosis. Case presentation We report the case of a 58-year-old man without any medical history who exhibited recurrent strokes in the same vascular territory (left internal carotid). Multiple biological screening, neuroimaging methods, and cardiovascular examinations failed to provide a diagnosis and treatment that could have prevented recurrences. Finally, B. burgdorferi sensu lato serology testing in blood and cerebrospinal fluid enabled diagnosis of LNB, in relation to a cerebral vasculitis. The patient experienced no further stroke after four weeks of doxycycline treatment. Conclusion B. burgdorferi central nervous system infection must be considered in case of unexplained recurrent and/or multiple strokes, especially if cerebral vasculitis is suspected or demonstrated on neuroimaging.
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7
Quantitative Polymerase Chain Reaction from Malaria Rapid Diagnostic Tests to Detect Borrelia crocidurae, the Agent of Tick-Borne Relapsing Fever, in Febrile Patients in Senegal
American Journal of Tropical Medicine and Hygiene, 3.05.2023
Tilføjet 3.05.2023
Journal Name: The American Journal of Tropical Medicine and Hygiene Volume: 108 Issue: 5 Pages: 968-976
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8
Dispatch - Borrelia miyamotoi Infection in Immunocompromised Man, California, USA, 2021
Emerging Infectious Diseases, 20.04.2023
Tilføjet 20.04.2023
Dispatch - Borrelia miyamotoi Infection in Immunocompromised Man, California, USA, 2021
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9
Borrelia burgdorferi Outer Surface Protein C Is Not the Sole Determinant of Dissemination in Mammals
Infection and Immunity, 7.03.2023
Tilføjet 7.03.2023
10
Biodiversity in the Lyme-light: ecological restoration and tick-borne diseases in Europe
Trends in Parasitology, 6.03.2023
Tilføjet 7.03.2023
Human cases of TBDs in Europe have increased steadily over the past decades [1]. Well-known pathogens such as Borrelia burgdorferi sensu lato and tick-borne encephalitis virus (TBEV) have expanded their distribution, while several others, such as Borrelia miyamotoi, have only recently been identified as human pathogens [2–4]. Although this upsurge can in part be explained by improved medical diagnostics and awareness, human demographics, and human behavior, it is also a consequence of environmental and climatic changes [5–7].
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11
Case Report: Exposure to Relapsing Fever Group Borreliae in Patients with Undifferentiated Febrile Illness in Mexico
American Journal of Tropical Medicine and Hygiene, 2.03.2023
Tilføjet 2.03.2023
Journal Name: The American Journal of Tropical Medicine and Hygiene Volume: 108 Issue: 3 Pages: 510-512
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12
Endogenous Linear Plasmids lp28-4 and lp25 Are Required for Infectivity and Restriction Protection in the Lyme Disease Spirochete Borrelia mayonii
Infection and Immunity, 28.02.2023
Tilføjet 1.03.2023
13
Role of the Hypothetical Protein BB0563 during Borrelia burgdorferi Infection in Animals
Infection and Immunity, 6.02.2023
Tilføjet 7.02.2023
14
Borrelia multiplex: a bead-based multiplex assay for the simultaneous detection of Borrelia specific IgG/IgM class antibodies
BMC Infectious Diseases, 18.11.2022
Tilføjet 18.11.2022
Abstract Background Lyme borreliosis (LB) is the most common tick-borne infectious disease in the northern hemisphere. The diagnosis of LB is usually made by clinical symptoms and subsequently supported by serology. In Europe, a two-step testing consisting of an enzyme-linked immunosorbent assay (ELISA) and an immunoblot is recommended. However, due to the low sensitivity of the currently available tests, antibody detection is sometimes inaccurate, especially in the early phase of infection, leading to underdiagnoses. Methods To improve upon Borrelia diagnostics, we developed a multiplex Borrelia immunoassay (Borrelia multiplex), which utilizes the new INTELLIFLEX platform, enabling the simultaneous dual detection of IgG and IgM antibodies, saving further time and reducing the biosample material requirement. In order to enable correct classification, the Borrelia multiplex contains eight antigens from the five human pathogenic Borrelia species known in Europe. Six antigens are known to mainly induce an IgG response and two antigens are predominant for an IgM response. Results To validate the assay, we compared the Borrelia multiplex to a commercial bead-based immunoassay resulting in an overall assay sensitivity of 93.7% (95% CI 84.8–97.5%) and a specificity of 96.5% (95%CI 93.5–98.1%). To confirm the calculated sensitivity and specificity, a comparison with a conventional 2-step diagnostics was performed. With this comparison, we obtained a sensitivity of 95.2% (95% CI 84.2–99.2%) and a specificity of 93.0% (95% CI 90.6–94.7%). Conclusion Borrelia multiplex is a highly reproducible cost- and time-effective assay that enables the profiling of antibodies against several individual antigens simultaneously.
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15
Persistent Borrelia burgdorferi Sensu Lato Infection after Antibiotic Treatment: Systematic Overview and Appraisal of the Current Evidence from Experimental Animal Models
Y. L. Verschoor,
A. Vrijlandt,
R. Spijker,
R. M. van Hest,
H. ter Hofstede,
K. van Kempen,
A. J. Henningsson,
J. W. Hovius
aAmsterdam UMC, Location University of Amsterdam, Department of Internal Medicine, Section of Infectious Diseases, Amsterdam UMC Multidisciplinary Lyme Borreliosis Center, Amsterdam, The Netherlands
bAmsterdam UMC, Location University of Amsterdam, Center for Experimental and Molecular Medicine, Amsterdam, The Netherlands
cAmsterdam UMC, Location University of Amsterdam, Amsterdam Public Health, Medical Library, Amsterdam, The Netherlands
dAmsterdam UMC, Location University of Amsterdam, Department of Hospital Pharmacy and Clinical Pharmacology, Amsterdam, The Netherlands
eDepartment of Internal Medicine, Section of Infectious Diseases, Lyme Borreliosis Outpatient Clinic, Radboudumc, Nijmegen, The Netherlands
fCoram NV, Pelt, Belgium
gDepartment of Biomedical and Clinical Sciences, Linköping University, Linköping, Sweden
hDepartment of Clinical Microbiology in Jönköping, Region Jönköping County, Linköping University, Linköping, Sweden
Clinical Microbiology Reviews, 12.10.2022
Tilføjet 12.10.2022
16
CspZ FH-Binding Sites as Epitopes Promote Antibody-Mediated Lyme Borreliae Clearance
Yi-Lin Chen,
Ashley L. Marcinkiewicz,
Tristan A. Nowak,
Rakhi Tyagi Kundu,
Zhuyun Liu,
Ulrich Strych,
Maria Elena Bottazzi,
Wen-Hsiang Chen,
Yi-Pin Lin
aDepartment of Pediatrics, National School of Tropical Medicine, Baylor College of Medicinegrid.39382.33, Houston, Texas, USA
bTexas Children’s Hospital Center for Vaccine Development, Houston, Texas, USA
cDivision of Infectious Diseases, Wadsworth Center, NYSDOH, Albany, New York, USA
dDepartment of Biomedical Sciences, SUNY Albany, Albany, New York, USA
eDepartment of Biology, Baylor University, Waco, Texas, USA,
De’Broski R. Herbert
Infection and Immunity, 5.07.2022
Tilføjet 5.07.2022
17
The false promise of cellular tests for Lyme borreliosis
Elitza S Theel, Bobbi S Pritt
Lancet Infectious Diseases, 15.06.2022
Tilføjet 15.06.2022
Lyme borreliosis, caused by members of the Borrelia burgdorferi sensu lato (Bbsl) complex, is the most common vector-borne disease in Europe and the USA, with over 600 000 cases estimated to occur annually in these regions since 2010.1,2 Given this impressive global burden, it is remarkable that there are still substantial diagnostic gaps for Lyme borreliosis, including the absence of reliable assays for acute infection, difficulty in differentiating past disease from current disease, and an inability to monitor treatment response.
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18
Multi-scale analysis of habitat fragmentation on small-mammal abundance and tick-borne pathogen infection prevalence in Essex County, MA
Samuel D. Mason, Samuel C. R. Sherratt, Samantha M. Kruguer, Michael Muthersbaugh, Jonathan P. Harris, Wayne C. Gatlin, Justin D. Topp, Gregory S. Keller
PLoS One Infectious Diseases, 13.06.2022
Tilføjet 13.06.2022
by Samuel D. Mason, Samuel C. R. Sherratt, Samantha M. Kruguer, Michael Muthersbaugh, Jonathan P. Harris, Wayne C. Gatlin, Justin D. Topp, Gregory S. Keller
Habitat fragmentation and heterogeneity transform otherwise contiguous tracks of forest into smaller patches in the northeastern U.S. and likely impact abundances, movement patterns, and disease transmission pathways for small-mammal communities at multiple scales. We sought to determine the structure of a small-mammal community in terms of mammal abundance and infection prevalence of Borrelia burgdorferi sensu stricto (s.s.), Anaplasma phagocytophilum, and Babesia microti within a fragmented landscape in Essex County, Massachusetts, USA. We studied communities at multiple spatial scales, including vegetation, edge type, and landscape (including 200-m, 500-m, and 1000-m radii) scales. A total of 16 study sites were chosen to represent four edge types: interior forest, pasture edge, natural edge, and residential edge. At each site, we trapped small mammals and conducted vegetation surveys and GIS analysis. Upon capture, a tissue sample was collected to analyze for presence of pathogens. Northern short-tailed shrew (Blarina brevicauda) abundance did not differ based on edge type, whereas abundance of the white-footed mouse (Peromyscus leucopus) was greatest at pasture edges, although the relationship was relatively weak. White-footed mouse abundance was negatively associated with amount of forested area within a 500-m radius, whereas northern short-tailed shrew abundance demonstrated a positive relationship with fragmentation indices at the 200-m radius. White-footed mice captured at interior-forest habitat were more likely be infected with B. burgdorferi (s.s.) than individuals from edge habitat. Greater prevalence of B. burgdorferi infection of white-footed mice in forest interiors compared to edge habitats counters previous studies. Reasons for this and implications are discussed.
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19
The diagnostic value of serum Borrelia burgdorferi antibodies and seroconversion after Lyme neuroborreliosis, a nationwide observational study
Malte M. Tetens, Ram Dessau, Svend Ellermann-Eriksen, Nanna S. Andersen, Charlotte Sværke Jørgensen, Christian Østergaard, Jacob Bodilsen, David F. Damgaard, Jette Bangsborg, Alex Christian Nielsen, Jens Kjølseth Møller, Lars Haukali Omland, Niels Obel, Anne-Mette Lebech
Clinical Microbiology and Infection, 13.06.2022
Tilføjet 13.06.2022
Clinical guidelines disagree on the diagnostic usefulness of Borrelia burgdorferi (Bb) serum antibodies (serum-Bb) in investigation of Lyme neuroborreliosis (LNB). We investigated the association between serum-Bb and Bb intrathecal antibody index (Bb-AI) and rates of seroconversion and seroreversion after LNB.
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20
A Borrelia burgdorferi outer surface protein C (OspC) genotyping method using Luminex technology
Patrick Pearson, Olivia Skaltsis, Chu-Yuan Luo, Guang Xu, Zachary Oppler, Dustin Brisson, Stephen M. Rich
PLoS One Infectious Diseases, 1.06.2022
Tilføjet 1.06.2022
by Patrick Pearson, Olivia Skaltsis, Chu-Yuan Luo, Guang Xu, Zachary Oppler, Dustin Brisson, Stephen M. Rich
Borrelia burgdorferi is an important tickborne human pathogen comprising several strains based on nucleotide sequence of the outer surface protein C (ospC) gene. Detection and characterization of different ospC genotypes is vital for research on B. burgdorferi and the risk it poses to humans. Here we present a novel, multiplex assay based on Luminex xMAP technology for the detection of B. burgdorferi ospC genotypes. The assay has five major steps: amplification of the ospC gene, hydrolyzation of surplus primers and nucleotides, incorporation of biotinylated nucleotides into the template DNA, hybridization to Luminex microspheres, and detection of fluorescent signals corresponding to each ospC genotype. We validated the protocol by comparing results obtained from our method against results from an established ospC genotyping method. This protocol can be used for the characterization of ospC genotypes in B. burgdorferi infected ticks, reservoir hosts, and/or clinical samples.
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21
Atypical Borrelia garinii infection in an immunocompromised patient mimicking high grade lymphoma
Victor Eiferman, Guillaume Le Guenno, Nathalie Boiret-Dupré, Bertrand Barres, Léa Luciani, Pierre Edouard Fournier
International Journal of Infectious Diseases, 4.05.2022
Tilføjet 4.05.2022